Check the pH on the supernatant mix with check paper ahead of proceeding. It ought to be about pH 5.five. Adjustment on the pH is vital for DNA binding to the silica column. If incomplete lysis is observed, enhance the incubation time or amount of Proteinase K used for lysis. https://www.facebook.com/NewTechFind/posts/pfbid02FAzSY5gdY3yCpunuLYa7Qgszx3z29VNdrfT9DiXP1S9C1kArgJ8LX4BvCkXyabnJl